Important Microbiology Interview Preparation Guide

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During growth promotion test, We check whether media is capable of promoting growth of inoculated microorganisms or not.So, in GPT culture suspension of 10-100 cfu's or not more than 100 cfu's required because first thing is that we can easily count the number of recovered microorganisms on the media plate.
Another important thing is that we are challenging the media with the microorganisms and the microorganisms which we use for GPT should not be more than 5 passage removed form the original culture. So, we are working with the healthy microorganisms and these microorganisms should grow on the media which we are testing. This is like a challenge to use low number of microorganisms to check the growth promotion properties of the media. For this reason we use 10-100 cfu's or not more than 100 cfu's for growth promotion test.

* A. Uncoating
* B. Virion Assembly
* C. Release by budding
* D. Adsorption
* E. Production of mRNA

Correct Answer: D

* A. True
* B. False

Correct Answer: A

* A. They contain both RNA and DNA
* B. The nucleic acid may be single or double stranded
* C. They are obligate intracellular parasites
* D. They reproduce using host cell energy
* E. The infectious particle is called a virion

Correct Answer: A

* A. True
* B. False

Correct Answer: B

* A. Conjuctivitis
* B. Coronary artery disease
* C. Myocarditis
* D. Pericarditis
* E. Pleuritis

Correct Answer: B

* A. There are many serotypes
* B. They can cause a variety of diseases which imitate bacterial infection
* C. They are an important cause of meningitis
* D. They are an important cause of diarrhea

Correct Answer: D

* A. Herpes simplex
* B. Hepatitis C
* C. Hepatitis A
* D. Rotavirus
* E. Rubella

Correct Answer: C

* A. Pyrophosphate-binding site of the polymerase
* B. Protease
* C. Nucleoside reductase
* D. Reverse transcriptase

Correct Answer: A

* A. Cleaves polyprotein precursor leading to functional assembly of viral core
* B. Cleaves sialic acid residue from glycoprotein permitting attachment and entry into cell
* C. Transcribes RNA into DNA
* D. Activates 2', 5’ oligoadenylate synthetase

Correct Answer: C

* A. Few drug targets, toxicity problematic
* B. Sensitivity tests of limited value
* C. Toxicity a minor problem, multiple enzymatic targets
* D. Many more drug options are available to treat viral infections

Correct Answer: A

* A. drug allergy
* B. antimetabolic effect on host cells due to lack of specificity for viral enzymes
* C. rate of development of drug resistance
* D. drug-drug interactions associated with competition for cytochrome P450 enzymes

Correct Answer: C

* A. In 2-3 days
* B. In 3-4 weeks
* C. In 3-6 months
* D. May take up to 10 years

Correct Answer: B

* A. The virus multiplies slowly with a half-life of weeks matching the slow rate of disease progression
* B. There is rapid dissemination of virus within the first few weeks of infection to all tissues
* C. In early infection the virus is most actively multiplying within the bloodstream
* D. There is little if any immune response to HIV infection, and that is why this infection is so overwhelming

Correct Answer: B

* A. 1 in 500
* B. 1 to 5 %
* C. 20 to 25%
* D. 50 to 75%

Correct Answer: C

* A. HTLV III
* B. HTLV II
* C. HTLV I
* D. None of the above

Correct Answer: A

* A. It is transmitted by eating raw oysters
* B. It is highly likely to cause chronic ongoing infection
* C. Most patients have severe symptoms when first infected
* D. It is a DNA virus

Correct Answer: B

* A. Hepatitis A
* B. Hepatitis B
* C. Hepatitis C
* D. Hepatitis A and B

Correct Answer: B

* A. Changing diapers on an infected baby
* B. Having sex with someone who is infected
* C. Eating cooked meat
* D. Needle sticks injuries from infected patients

Correct Answer: A

* A. Fever, nausea, diarrhea
* B. Malaise, anorexia, jaundice
* C. Nausea, vomiting, right upper quadrant abdominal pain
* D. Clear urine, dark colored stools, anorexia

Correct Answer: B

* A. Enteroviruses
* B. Rhabdoviruses
* C. California/Lacrosse virus
* D. Lymphocytic choriomeningitis virus

Correct Answer: A

* A. Astrocytes
* B. Oligodendrocytes
* C. Neurons
* D. Macrophages

Correct Answer: C

* A. JC virus
* B. Pumuula virus
* C. Prions
* D. SV40 virus

Correct Answer: C

* A. Hematogenous distribution
* B. Neurotropic spread
* C. Entry into brain within macrophages
* D. Both "A" and "B"

Correct Answer: B