Biochemistry Interview Preparation Guide
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Biochemistry Interview Question will guide you now that Biochemistry sometimes called biological chemistry, is the study of chemical processes in living organisms, including, but not limited to, living matter. Biochemistry governs all living organisms and living processes. By controlling information flow through biochemical signalling and the flow of chemical energy through metabolism.

22 Biochemistry Questions and Answers:

1 :: Explain What is the primary structure of a protein? What is the importance of the primary structure?

The primary protein structure is the linear sequence of amino acids that form the molecule.

The primary structure is the basis of the protein identity. Modification of only one amino acid of the primary structure creates a different protein. This different protein can be inactive or even can have other biological function.

Protein Structure Review - Image Diversity: protein primary structure

2 :: Explain heat of Combustion?

The amount of heat liberated when one mole of any substance is completely burnt in oxygen is called heat of combustion H is negative for heat of combustion.

3 :: Explain What does Thermodynamics helps in predicting?

Predicting the feasibility of reaction and does not indicate the rate of chemical reaction

4 :: Explain and Define standard feat of formation

The amount of heat liberated of absorbed when one mole of compound is formed in its standard state from its elements in their standard state is called standard heat of formation ( Hf *)

6 :: What are pentoses? To what organic group do pentoses belong? Are nucleotides formed of only one type of pentose?

Pentoses are carbohydrates made of five carbons. Deoxyribose is the pentose that constitutes DNA nucleotides and ribose is the pentose that is part of RNA nucleotides.

7 :: Explain Why can it be said that the enzymatic action is highly specific?

The enzymatic action is highly specific because only specific substrates of one enzyme bind to the activation center of that enzyme. Each enzyme generally catalyzes only a specific chemical reaction.

8 :: Explain kararch effect?

In the addition of the hydrogen, halide to unsaturated alkenes in the presence of peroxides the halide adds to the carbon atom linked to more number of hydrogen atoms and hydrogen adds to the carbon atom linked to lesser number of hydrogen atoms. This is called Peroxide effect (or) Kharasch effect.


9 :: Concerning their biological function what is the difference between RNA and DNA?

DNA is the source of information for RNA production (transcription) and thus for protein synthesis. DNA is still the basis of heredity due to its replication capability.

The messenger RNA is the template for protein synthesis (translation). In this process, tRNA and rRNA also participate since the first carries amino acids for the polypeptide chain formation and the second is a structural constituent of ribosomes (the organelles where proteins are made).

10 :: Explain What is the name of the DNA duplication process? What is the main enzyme that participates in it?

The process of copying, or duplication, of the DNA molecule is called replication. The enzyme that participates in the formation of a new DNA chain is the DNA polymerase. There are also other important enzymes in the replication process, the helicase, the gyrase and the ligase.

Nucleic Acid Review - Image Diversity: DNA replication