Bio Technology Interview Questions & Answers

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rna is having _ve charge & DBM paper having positive chrg
hence due to thease opposite charges DBM paper binds to RNA

positively charged i.e,cationic nature

diazobenzyloxymethyl paper that binds all single-stranded DNA, RNA, and proteins by means of covalent linkages to the diazonium group; used in situations where nitrocellulose blotting is not technically feasible

it is used to determine the protein from a mixture of compounds

eukaryotes does not contain restriction modificayion system

Transfection- (Transfer+ Infection)transfer of naked DNA
(DNA without any carrier)into an Eukaryotic cell.
Transduction-Transfer of genetic material among bacteria by
carrier (bacteriophage).
Since the term 'Transformation' is used in case of transfer
of naked DNA (through non viral particles) into
prokaryotes,while the term 'Transfection' is uused in case
of eukaryotes because the term transformation is used for
the conversion of normal body cells into cancerous cells
which undergo unlimited cell division, so to avoid the
confusion between these two different cell processes the
other term Transfection is used in eukaryotes in place of
the term transformation in prokaryotes.

Transfection:Transfer of naked dna into eukaryotic cell

Transduction:Trnsfer of genetic material from bacteria to
virus through bacteriophage

Why, bacteria eat almost anything they can get! Of course,
they can't chew through cement. They can eat anything
edible to animals and humans, probably more! Take wood for
example, termites eat wood, bacteria eat wood too. In fact,
termites wouldn't even be able to eat wood if it wasn't for
the bacteria in their stomachs!

Bacteria are decomposers. Decomposers eat anything dead and
once they are finished they put nutrients from the dead
object or plant back into the ground to grow new plants

define serum is the artificial serum made by man and
composition of which is well known by us
characterized serum is that which made up of specific
characters for example it contains specific antibody against
specific disease
stander serum is serum which is collected from normal blood
which we can not make in laboratory scale although we know
composition of it.

1- In drug discovery research

2- Analysis of genome wide biomedical data

3- In genome and sequence analysis

By genetically modifying any plant such that it will resist
the climate in dubai n still will give fruits n green
leaves....
for eg...
may be cutting a gene code from cactus plant and pasting it
in apple plant...

PhD is a threshold qualification about research, & makes capable to present your idea/view as a Philosopher.

The father of term biotechnology is Karl Ereky and father of
biotechnology is Louis Pasteur

1- restriction enzyme
2- ligase enzyme
3- formation of recombintant plasmid by using these enzyme
4- selection of transformed cell
5- cDNA and genomic library

Glucose is reactive because its open-chain form contains an
aldehyde group.

no

by determination of optical density by coloimetric method