Bio Technology Question: Download Questions PDF
What is the difference between transfection and transduction?why the word trasfection is prefers for transfer of genetic material through non viral particles in eukaryote not for prokaryote?
Answer:
Transfection- (Transfer+ Infection)transfer of naked DNA
(DNA without any carrier)into an Eukaryotic cell.
Transduction-Transfer of genetic material among bacteria by
carrier (bacteriophage).
Since the term 'Transformation' is used in case of transfer
of naked DNA (through non viral particles) into
prokaryotes,while the term 'Transfection' is uused in case
of eukaryotes because the term transformation is used for
the conversion of normal body cells into cancerous cells
which undergo unlimited cell division, so to avoid the
confusion between these two different cell processes the
other term Transfection is used in eukaryotes in place of
the term transformation in prokaryotes.
(DNA without any carrier)into an Eukaryotic cell.
Transduction-Transfer of genetic material among bacteria by
carrier (bacteriophage).
Since the term 'Transformation' is used in case of transfer
of naked DNA (through non viral particles) into
prokaryotes,while the term 'Transfection' is uused in case
of eukaryotes because the term transformation is used for
the conversion of normal body cells into cancerous cells
which undergo unlimited cell division, so to avoid the
confusion between these two different cell processes the
other term Transfection is used in eukaryotes in place of
the term transformation in prokaryotes.
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