Electron Microscopist Interview Questions And Answers

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Optimize your Electron Microscopist interview preparation with our curated set of 50 questions. Our questions cover a wide range of topics in Electron Microscopist to ensure you're well-prepared. Whether you're new to the field or have years of experience, these questions are designed to help you succeed. Download the free PDF to have all 50 questions at your fingertips. This resource is designed to boost your confidence and ensure you're interview-ready.

50 Electron Microscopist Questions and Answers:

Electron Microscopist Job Interview Questions Table of Contents:

Electron Microscopist Job Interview Questions and Answers

1 :: The substage condenser:
A) changes the wavelength of the light reaching the specimen
B) focuses light on the specimen
C) decreases the amount of light reaching the specimen

B) focuses light on the specimen

2 :: A 40X objective and an 10X ocular produce a total magnification of:
A) 50
B) 400
C) 90

B) 400

3 :: Living, unstained cells and organisms can be observed best using:
A) fluorescent microscopy
B) TEM
C) phase contrast microscopy

C) phase contrast microscopy

4 :: Scanning electron microscopy (SEM) is best used to study
A) small internal cell structures.
B) surface morphology.
C) all of the above

B) surface morphology.

5 :: A microscope in which an image is formed by passing an electron beam through a specimen and focusing the scattered electrons with magnetic lenses is called a:
A) transmission electron microscope
B) scanning electron microscope
C) phase-contrast microscope

A) transmission electron microscope

6 :: Resolution is the ability of a lens to distinguish between small objects close together. What approximate resolution can be obtained with a lower power (10X, N.A. 0.25) objective lens?
A) 0.2 microns
B) 0.3 microns
C) 0.9 microns
D) 0.25

C) 0.9 microns

7 :: Fixation is the process by which the structures of the cells are preserved and fixed in position. An advantage of chemical fixation over heat fixation is that it:
A) magnifies the specimen
B) does not destroy internal structures

B) does not destroy internal structures

8 :: Monochromatic (one color) light is sometimes used to increase the resolution of light microscopes. Light of which color below would give you the best resolution?
A) Red
B) Orange
C) Green
D) Blue

D) Blue

9 :: Which of the following objectives would give you the best resolution of small objects?
A) 10x air, N.A. 0.25
B) 40x air, N.A. 0.65
C) 64x oil, N.A. 1.4
D) 100x oil, N.A. 1.25

C) 64x oil, N.A. 1.4

10 :: Transmission electron microscopy is best for high magnification viewing of:
A) internal structure of fixed cells.
B) internal structure of live, motile cells.
C) surface structure of fixed cells.
D) surface membranes of live, motile cells

A) internal structure of fixed cells.

11 :: Which of the following statements is most correct about the differential Gram stain?
A) Crystal violet differentially stains Gram positive cells.
B) Gram's iodine differentially stains Gram positive cells.
C) Acetone differentially destains Gram negative cells.
D) Saffron red differentially stains Gram negative cells

C) Acetone differentially destains Gram negative cells.

12 :: Which of the following statements is most correct about Atomic Force Microscopy (AFM)?
A) AFM can visualize protein bound to DNA molecules.
B) AFM can visualize unfixed specimens in water or buffer.
C) AFM moves a very sharp tip over the surface of the specimen to "feel" its shape.
D) All the statements above are true

D) All the statements above are true

13 :: Which of the following statements about Transmission Electron Microscopy is not true.
A) The specimen must be stained with osmium or other heavy metal.
B) The specimens are placed in a high vacuum for viewing.
C) The specimens must be sliced very thin, 20-100 nm in thickness.
D) The beam is focused by electromagnetic lenses

A) The specimen must be stained with osmium or other heavy metal.

14 :: Phase Contrast microscopy:
A) Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen.
B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices.
C) Uses special lenses to distinguish between solid and liquid phases of the cell.
D) Uses special lenses to change the color of light passing through them

B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices.

15 :: Which of the following is NOT equivalent to 10 micrometers.
A) 0.0001 cm
B) 0.01 mm
C) 10,000 nm
D) 100,000 Angstroms

A) 0.0001 cm

16 :: "Parfocal" refers to microscopes with multiple objectives where:
A) objectives are used in pairs for stereoscopic effects.
B) each objective has the same working distance above the specimen.
C) each objective is positioned to be in focus at the same stage height.
D) sequential objectives increase power by a factor of two.

C) each objective is positioned to be in focus at the same stage height.

17 :: Differential Interference Contrast microscopy
A) compares two identical specimens on the same microscope.
B) illuminates the specimen with light of two different colors.
C) illuminates the specimen with light of two different phases.
D) illuminates the specimen with both reflected and transmitted light

C) illuminates the specimen with light of two different phases.

18 :: In a compound microscope, the primary image is formed by an objective lens and enlarged by the optical lens to reveal a virtual image.
A) True
B) False

A) True

19 :: Microscope resolution increases as the wavelength of radiation used to illuminate the specimen decreases.
A) True
B) False

A) True

20 :: The fluorescence microscope has become an essential tool in medical microbiology to identify bacterial pathogens.
A) True
B) False

A) True

21 :: Objective lenses with higher magnification generally have a shorter working distance.
A) True
B) False

A) True

22 :: Even on the best light microscopes, increasing total magnification beyond 1500 cannot result in better resolution of the image.
A) True
B) False

A) True

23 :: Darkfield microscopy is best for visualizing live, motile microorganisms.
A) True
B) False

A) True

24 :: Fluorescence microscopy must be done on fixed and stained microorganisms.
A) True
B) False

B) False

25 :: Fluorescence microscopy may be used with specially stained antibodies to detect specific microorganisms.
A) True
B) False

A) True

Electron Microscopist Interview Questions And Answers

Download Electron Microscopist Interview Questions and Answers PDF

50 Electron Microscopist Questions and Answers:

Electron Microscopist Job Interview Questions Table of Contents:

1 :: The substage condenser: A) changes the wavelength of the light reaching the specimen B) focuses light on the specimen C) decreases the amount of light reaching the specimen

2 :: A 40X objective and an 10X ocular produce a total magnification of: A) 50 B) 400 C) 90

3 :: Living, unstained cells and organisms can be observed best using: A) fluorescent microscopy B) TEM C) phase contrast microscopy

4 :: Scanning electron microscopy (SEM) is best used to study A) small internal cell structures. B) surface morphology. C) all of the above

5 :: A microscope in which an image is formed by passing an electron beam through a specimen and focusing the scattered electrons with magnetic lenses is called a: A) transmission electron microscope B) scanning electron microscope C) phase-contrast microscope

6 :: Resolution is the ability of a lens to distinguish between small objects close together. What approximate resolution can be obtained with a lower power (10X, N.A. 0.25) objective lens? A) 0.2 microns B) 0.3 microns C) 0.9 microns D) 0.25

7 :: Fixation is the process by which the structures of the cells are preserved and fixed in position. An advantage of chemical fixation over heat fixation is that it: A) magnifies the specimen B) does not destroy internal structures

8 :: Monochromatic (one color) light is sometimes used to increase the resolution of light microscopes. Light of which color below would give you the best resolution? A) Red B) Orange C) Green D) Blue

9 :: Which of the following objectives would give you the best resolution of small objects? A) 10x air, N.A. 0.25 B) 40x air, N.A. 0.65 C) 64x oil, N.A. 1.4 D) 100x oil, N.A. 1.25

10 :: Transmission electron microscopy is best for high magnification viewing of: A) internal structure of fixed cells. B) internal structure of live, motile cells. C) surface structure of fixed cells. D) surface membranes of live, motile cells

15 :: Which of the following is NOT equivalent to 10 micrometers. A) 0.0001 cm B) 0.01 mm C) 10,000 nm D) 100,000 Angstroms

18 :: In a compound microscope, the primary image is formed by an objective lens and enlarged by the optical lens to reveal a virtual image. A) True B) False

19 :: Microscope resolution increases as the wavelength of radiation used to illuminate the specimen decreases. A) True B) False

20 :: The fluorescence microscope has become an essential tool in medical microbiology to identify bacterial pathogens. A) True B) False

21 :: Objective lenses with higher magnification generally have a shorter working distance. A) True B) False

22 :: Even on the best light microscopes, increasing total magnification beyond 1500 cannot result in better resolution of the image. A) True B) False

23 :: Darkfield microscopy is best for visualizing live, motile microorganisms. A) True B) False

24 :: Fluorescence microscopy must be done on fixed and stained microorganisms. A) True B) False

25 :: Fluorescence microscopy may be used with specially stained antibodies to detect specific microorganisms. A) True B) False

1 :: The substage condenser:
A) changes the wavelength of the light reaching the specimen
B) focuses light on the specimen
C) decreases the amount of light reaching the specimen

2 :: A 40X objective and an 10X ocular produce a total magnification of:
A) 50
B) 400
C) 90

3 :: Living, unstained cells and organisms can be observed best using:
A) fluorescent microscopy
B) TEM
C) phase contrast microscopy

4 :: Scanning electron microscopy (SEM) is best used to study
A) small internal cell structures.
B) surface morphology.
C) all of the above

5 :: A microscope in which an image is formed by passing an electron beam through a specimen and focusing the scattered electrons with magnetic lenses is called a:
A) transmission electron microscope
B) scanning electron microscope
C) phase-contrast microscope

6 :: Resolution is the ability of a lens to distinguish between small objects close together. What approximate resolution can be obtained with a lower power (10X, N.A. 0.25) objective lens?
A) 0.2 microns
B) 0.3 microns
C) 0.9 microns
D) 0.25

7 :: Fixation is the process by which the structures of the cells are preserved and fixed in position. An advantage of chemical fixation over heat fixation is that it:
A) magnifies the specimen
B) does not destroy internal structures

8 :: Monochromatic (one color) light is sometimes used to increase the resolution of light microscopes. Light of which color below would give you the best resolution?
A) Red
B) Orange
C) Green
D) Blue

9 :: Which of the following objectives would give you the best resolution of small objects?
A) 10x air, N.A. 0.25
B) 40x air, N.A. 0.65
C) 64x oil, N.A. 1.4
D) 100x oil, N.A. 1.25

10 :: Transmission electron microscopy is best for high magnification viewing of:
A) internal structure of fixed cells.
B) internal structure of live, motile cells.
C) surface structure of fixed cells.
D) surface membranes of live, motile cells

15 :: Which of the following is NOT equivalent to 10 micrometers.
A) 0.0001 cm
B) 0.01 mm
C) 10,000 nm
D) 100,000 Angstroms

18 :: In a compound microscope, the primary image is formed by an objective lens and enlarged by the optical lens to reveal a virtual image.
A) True
B) False

19 :: Microscope resolution increases as the wavelength of radiation used to illuminate the specimen decreases.
A) True
B) False

20 :: The fluorescence microscope has become an essential tool in medical microbiology to identify bacterial pathogens.
A) True
B) False

21 :: Objective lenses with higher magnification generally have a shorter working distance.
A) True
B) False

22 :: Even on the best light microscopes, increasing total magnification beyond 1500 cannot result in better resolution of the image.
A) True
B) False

23 :: Darkfield microscopy is best for visualizing live, motile microorganisms.
A) True
B) False

24 :: Fluorescence microscopy must be done on fixed and stained microorganisms.
A) True
B) False

25 :: Fluorescence microscopy may be used with specially stained antibodies to detect specific microorganisms.
A) True
B) False