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What is use of acetonitrile compare to methanol in RP-HPLC Method development?
Answers:
Answer #1ACN is highly polar as compare to Methanol So provide Better
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.
Answer #2Acetonitrile has a higher elution strength than methanol for reversed-phase chromatography, therefore shorter analyte retention can be expected for equal proportions of organic to water
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