Electron Microscopist Question:

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Phase Contrast microscopy:
A) Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen.
B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices.
C) Uses special lenses to distinguish between solid and liquid phases of the cell.
D) Uses special lenses to change the color of light passing through them

Electron Microscopist Interview Question
Electron Microscopist Interview Question

Answer:

B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices.

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Which of the following statements about Transmission Electron Microscopy is not true.
A) The specimen must be stained with osmium or other heavy metal.
B) The specimens are placed in a high vacuum for viewing.
C) The specimens must be sliced very thin, 20-100 nm in thickness.
D) The beam is focused by electromagnetic lenses
Which of the following is NOT equivalent to 10 micrometers.
A) 0.0001 cm
B) 0.01 mm
C) 10,000 nm
D) 100,000 Angstroms