Bioinformatics Question: Download Bioinformatics PDF
How do you customise database for blast?
Answer:
Step 1. Assemble your sequences.
Put all your sequences in Fasta format in a single file.
This file should be located in a suitably named
subdirectory of your home directory on the UBiC Blast
server. The definition line for each sequence should start
with a unique identifier for that sequence.
Step 2. Convert this sequence file to a Blastable database.
The command formatdb converts your Fasta file of sequences
to a set of files that can be queried with command-line
BLAST.
Step 3. Test Blast on your database.
See the UBiC tutorial, Using Command-line BLAST. In the
blastall command-line you will need to specify the location
of your database by typing: -
d /disk2/home/myhome/blastdbs/custom.aa.
Put all your sequences in Fasta format in a single file.
This file should be located in a suitably named
subdirectory of your home directory on the UBiC Blast
server. The definition line for each sequence should start
with a unique identifier for that sequence.
Step 2. Convert this sequence file to a Blastable database.
The command formatdb converts your Fasta file of sequences
to a set of files that can be queried with command-line
BLAST.
Step 3. Test Blast on your database.
See the UBiC tutorial, Using Command-line BLAST. In the
blastall command-line you will need to specify the location
of your database by typing: -
d /disk2/home/myhome/blastdbs/custom.aa.
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