Bacteriologist Question:

Explain me what Are Cold Methods Of Acid Fast Staining?

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Answer:

The two methods namely Kinyoun’s and Gabbetts dont involve heating of slides, hence called cold methods. Heating is substituted by increased concentration of phenol and prolonging the duration of staining. Kinyoun’s method is favoured for detection of Cryptosporidium oocysts in fecal samples. Gabbetts method has decolourizer and counterstain in one solution.

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